Preparation of therapy-grade extracellular vesicles from adipose tissue to promote diabetic wound healing.

ABSTRACT: Background: Treatment of diabetic wounds is a major challenge in clinical practice. Extracellular vesicles (EVs) from adipose-derived stem cells have shown effectiveness in diabetic wound models. However, obtaining ADSC-EVs requires culturing vast numbers of cells, which is hampered by the need for expensive equipment and reagents, extended time cost, and complicated procedures before commercialization. Therefore, methods to extract EVs from discarded tissue need to be developed, for immediate application during surgery. For this reason, mechanical, collagenase-digestive, and constant in-vitro-collective methods were designed and compared for preparing therapy-grade EVs directly from adipose tissue. Methods: Characteristics and quantities of EVs were detected by transm

SUBMITTER: Pan C 

PROVIDER: S-EPMC10076785 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature