Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free P_DH promoter.

ABSTRACT: The current transition towards the circular bioeconomy requires a rational development of biorefineries to sustainably fulfill the present demands. The use of Komagataella phaffii (Pichia pastoris) can meet this challenge, since it has the capability to use crude glycerol as a carbon-source, a by-product from the biodiesel industry, while producing high- and low-added value products. Recombinant protein production (RPP) using K. phaffii has often been driven either by the methanol induced AOX1 promoter (P _AOX1 ) and/or the constitutive GAP promoter (P _GAP ). In the last years, strong efforts have been focused on developing novel expression systems that expand the toolbox variety of K. phaffii to efficiently produ

SUBMITTER: Bernat-Camps N 

PROVIDER: S-EPMC10076887 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature