Rapid, Direct, Noninvasive Method to Determine the Amount of Immobilized Protein.

ABSTRACT: Protein immobilization is of utmost importance in many areas, where various proteins are used for selective detection of target compounds. Despite the importance given to determine the amount of immobilized protein, there is no simple method that allows direct, noninvasive detection. In this work, a method based on pH transition, occurring during change of solution ionic strength, was developed. The method utilized the ionic character of the immobilized protein while implementing biologically compatible buffers. Five different proteins, namely, glucose oxidase, horseradish peroxidase, bovine serum albumin, lysozyme, and protein A, were immobilized in different amounts on a porous polymeric matrix, and their pH transition was measured using lactate buffer of various concentrations and pH va

SUBMITTER: Ambrozic R 

PROVIDER: S-EPMC10077329 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature