Application of TraDIS to define the core essential genome of Campylobacter jejuni and Campylobacter coli.

ABSTRACT: Campylobacter species are the major cause of bacterial gastroenteritis. As there is no effective vaccine, combined with the rapid increase in antimicrobial resistant strains, there is a need to identify new targets for intervention. Essential genes are those that are necessary for growth and/or survival, making these attractive targets. In this study, comprehensive transposon mutant libraries were created in six C. jejuni strains, four C. coli strains and one C. lari and C. hyointestinalis strain, allowing for those genes that cannot tolerate a transposon insertion being called as essential. Comparison of essential gene lists using core genome analysis can highlight those genes which are common across multiple strains and/or species. Comparison of C. jejuni and C. coli, the two species tha

SUBMITTER: Stoakes E 

PROVIDER: S-EPMC10077673 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature