A simple goniometer-compatible flow cell for serial synchrotron X-ray crystallography.

ABSTRACT: Serial femtosecond crystallography was initially developed for room-temperature X-ray diffraction studies of macromolecules at X-ray free electron lasers. When combined with tools that initiate biological reactions within microcrystals, time-resolved serial crystallography allows the study of structural changes that occur during an enzyme catalytic reaction. Serial synchrotron X-ray crystallography (SSX), which extends serial crystallography methods to synchrotron radiation sources, is expanding the scientific community using serial diffraction methods. This report presents a simple flow cell that can be used to deliver microcrystals across an X-ray beam during SSX studies. This device consists of an X-ray transparent glass capillary mounted on a goniometer-compatible 3D-printed support an

SUBMITTER: Ghosh S 

PROVIDER: S-EPMC10077854 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature