Hepatocyte-specific knockout of HIF-2α cannot alleviate carbon tetrachloride-induced liver fibrosis in mice.

ABSTRACT:

Background

The effects of hypoxia inducible factor-2α (HIF-2α) deficiency on liver fibrosis have not been demonstrated in a fibrosis model induced by carbon tetrachloride (CCl₄). We aimed to examine whether hepatocyte-specific HIF-2α deletion could ameliorate CCl₄-induced liver fibrosis in mice.

Methods

Hepatocyte-specific HIF-2α knockout mice were created using an albumin promoter-driven Cre recombinase. HIF-2α knockout (KO) mice and floxed control wild-type (WT) mice were fed a normal diet (ND) and received either twice weekly intraperitoneal injections of CCl₄ solution (CCl₄ dissolved in olive oil) or the corresponding amount of olive oil for 8 weeks. The indicators of liver function, glucose and lipid metabolism, and liver hist

SUBMITTER: Ye J 

PROVIDER: S-EPMC10078453 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature