Tissue optimization strategies for high-quality ex vivo diffusion imaging.

ABSTRACT: Ex vivo diffusion imaging can be used to study healthy and pathological tissue microstructure in the rodent brain with high resolution, providing a link between in vivo MRI and ex vivo microscopy techniques. Major challenges for the successful acquisition of ex vivo diffusion imaging data however are changes in the relaxivity and diffusivity of brain tissue following perfusion fixation. In this study we address this question by examining the combined effects of tissue preparation factors that influence signal-to-noise ratio (SNR) and consequently image quality, including fixative concentration, contrast agent concentration and tissue rehydration time. We present an optimization strategy combining these factors to manipulate the T1 and T2 of fixed tissue and maximize SNR efficiency. We appl

SUBMITTER: Barrett RLC 

PROVIDER: S-EPMC10078604 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature