Stability of End-to-End Base Stacking Interactions in Highly Concentrated DNA Solutions.

ABSTRACT: Positionally ordered bilayer liquid crystalline nanostructures formed by gapped DNA (GDNA) constructs provide a practical window into DNA-DNA interactions at physiologically relevant DNA concentrations; concentrations several orders of magnitude greater than those in commonly used biophysical assays. The bilayer structure of these states of matter is stabilized by end-to-end base stacking interactions; moreover, such interactions also promote in-plane positional ordering of duplexes that are separated from each other by less than twice the duplex diameter. The end-to-end stacked as well as in-plane ordered duplexes exhibit distinct signatures when studied via small-angle X-ray scattering (SAXS). This enables analysis of the thermal stability of both the end-to-end and side-by-side interact

SUBMITTER: Kodikara SG 

PROVIDER: S-EPMC10078606 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature