Eight key rules for successful data-dependent acquisition in mass spectrometry-based metabolomics.

ABSTRACT: In recent years, metabolomics has emerged as a pivotal approach for the holistic analysis of metabolites in biological systems. The rapid progress in analytical equipment, coupled to the rise of powerful data processing tools, now provides unprecedented opportunities to deepen our understanding of the relationships between biochemical processes and physiological or phenotypic conditions in living organisms. However, to obtain unbiased data coverage of hundreds or thousands of metabolites remains a challenging task. Among the panel of available analytical methods, targeted and untargeted mass spectrometry approaches are among the most commonly used. While targeted metabolomics usually relies on multiple-reaction monitoring acquisition, untargeted metabolomics use either data-independent acq

SUBMITTER: Defossez E 

PROVIDER: S-EPMC10078780 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature