Automated optimisation of solubility and conformational stability of antibodies and proteins.

ABSTRACT: Biologics, such as antibodies and enzymes, are crucial in research, biotechnology, diagnostics, and therapeutics. Often, biologics with suitable functionality are discovered, but their development is impeded by developability issues. Stability and solubility are key biophysical traits underpinning developability potential, as they determine aggregation, correlate with production yield and poly-specificity, and are essential to access parenteral and oral delivery. While advances for the optimisation of individual traits have been made, the co-optimization of multiple traits remains highly problematic and time-consuming, as mutations that improve one property often negatively impact others. In this work, we introduce a fully automated computational strategy for the simultaneous optimisation

SUBMITTER: Rosace A 

PROVIDER: S-EPMC10079162 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature