An improved method for determining frequency of multiple variants of SARS-CoV-2 in wastewater using qPCR assays.

ABSTRACT: Wastewater-based surveillance has become an effective tool around the globe for indirect monitoring of COVID-19 in communities. Variants of Concern (VOCs) have been detected in wastewater by use of reverse transcription polymerase chain reaction (RT-PCR) or whole genome sequencing (WGS). Rapid, reliable RT-PCR assays continue to be needed to determine the relative frequencies of VOCs and sub-lineages in wastewater-based surveillance programs. The presence of multiple mutations in a single region of the N-gene allowed for the design of a single amplicon, multiple probe assay, that can distinguish among several VOCs in wastewater RNA extracts. This approach which multiplexes probes designed to target mutations associated with specific VOC's along with an intra-amplicon universal probe (non-m

SUBMITTER: Fuzzen M 

PROVIDER: S-EPMC10079313 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature