Dataset Information

Atomic force and infrared spectroscopic studies on the role of surface charge for the anti-biofouling properties of polydopamine films.

ABSTRACT: Antibacterial polymer materials have gained interest due to their capability to inhibit or eradicate biofilms with greater efficiency in comparison with their monomeric counterparts. Among the antimicrobial and anti-biofouling polymers, catecholamine-based polymers - and in particular polydopamine - have been studied due to their favorable adhesion properties, which can be tuned by controlling the pH value. In this study, we used atomic force microscopy (AFM)-based spectroscopy to investigate the relation between the adhesion properties and surface charge density and the pH of electrochemically deposited polydopamine films presenting a dissociation constant of polydopamine of 6.3 ± 0.2 and a point of zero charge of 5.37 ± 0.06. Furthermore, using AFM and attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), the influence of the surface charge density of polydopamine on bacterial adhesion and biofilm formation was investigated. It was shown that the adhesion of Escherichia coli at positively charged polydopamine is three times higher compared to a negatively charged polymer, and that the formation of biofilms is favored at positively charged polymers.

SUBMITTER: Caniglia G

PROVIDER: S-EPMC10079710 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Atomic force and infrared spectroscopic studies on the role of surface charge for the anti-biofouling properties of polydopamine films.

Caniglia Giada G Teuber Andrea A Barth Holger H Mizaikoff Boris B Kranz Christine C

Analytical and bioanalytical chemistry 20221124 11

Antibacterial polymer materials have gained interest due to their capability to inhibit or eradicate biofilms with greater efficiency in comparison with their monomeric counterparts. Among the antimicrobial and anti-biofouling polymers, catecholamine-based polymers - and in particular polydopamine - have been studied due to their favorable adhesion properties, which can be tuned by controlling the pH value. In this study, we used atomic force microscopy (AFM)-based spectroscopy to investigate th ...[more]

PMID: 36434170

Similar Datasets

Project description:Biofouling of polymeric membranes is a severe problem in water desalination and treatment applications. A fundamental understanding of biofouling mechanisms is necessary to control biofouling and develop more efficient mitigation strategies. To shed light on the type of forces that govern the interactions between biofoulants and membranes, biofoulant-coated colloidal AFM probes were employed to investigate the biofouling mechanisms of two model biofoulants, BSA and HA, toward an array of polymer films commonly used in membrane synthesis, which included CA, PVC, PVDF, and PS. These experiments were combined with quartz crystal microbalance with dissipation monitoring (QCM-D) measurements. The Derjaguin, Landau, Verwey, and Overbeek (DLVO) and the extended-DLVO (XDLVO) theoretical models were applied to decouple the overall adhesion interactions between the biofoulants and the polymer films into their component interactions, i.e., electrostatic (El), Lifshitz-van der Waals (LW), and Lewis acid-base (AB) interactions. The XDLVO model was found to predict better the AFM colloidal probe adhesion data and the QCM-D adsorption behavior of BSA onto the polymer films than the DLVO model. The ranking of the polymer films' adhesion strengths and adsorption quantities was inversely proportional to their γ- values. Higher normalized adhesion forces were quantified for the BSA-coated colloidal probes with the polymer films than the HA-coated colloidal probes. Similarly, in QCM-D measurements, BSA was found to cause larger adsorption mass shifts, faster adsorption rates, and more condensed fouling layers than HA. A linear correlation (R2 = 0.96) was obtained between the adsorption standard free energy changes (ΔGads°) estimated for BSA from the equilibrium QCM-D adsorption experiments and the AFM normalized adhesion energies (WAFM/R) estimated for BSA from the AFM colloidal probe measurements. Eventually, an indirect approach was presented to calculate the surface energy components of biofoulants characterized by high porosities from Hansen dissolution tests to perform the DLVO/XDLVO analyses.

Dataset Information

Atomic force and infrared spectroscopic studies on the role of surface charge for the anti-biofouling properties of polydopamine films.

Publications

Atomic force and infrared spectroscopic studies on the role of surface charge for the anti-biofouling properties of polydopamine films.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets