Leptin deficiency-caused behavioral change - A comparative analysis using EthoVision and DeepLabCut.

ABSTRACT:

Introduction

Obese rodents e.g., the leptin-deficient (ob/ob) mouse exhibit remarkable behavioral changes and are therefore ideal models for evaluating mental disorders resulting from obesity. In doing so, female as well as male ob/ob mice at 8, 24, and 40 weeks of age underwent two common behavioral tests, namely the Open Field test and Elevated Plus Maze, to investigate behavioral alteration in a sex- and age dependent manner. The accuracy of these tests is often dependent on the observer that can subjectively influence the data.

Methods

To avoid this bias, mice were tracked with a video system. Video files were further analyzed by the compared use of two software, namely EthoVision (EV) and DeepLabCut (DLC). In DLC a Deep Learning application forms the basis for using art

SUBMITTER: Buhler D 

PROVIDER: S-EPMC10079875 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature