CRISPR/Cas9 establishment-mediated targeted mutagenesis in Macrobrachium nipponense.

ABSTRACT: Introduction: CRISPR/Cas9 is a gene-editing technology which could specifically cleave dsDNA and induce target gene mutation. CRISPR/Cas9 has been widely used in gene functional studies in many fields, such as medicine, biology, and agriculture due to its simple design, low cost, and high efficiency. Although it has been well developed in model fish and freshwater fish for gene function analysis, it is still novel in the studies dealing with economic crustacean species. Methods: In this study, we established a CRISPR/Cas9 system based on microinjection for M. nipponense, an important economic crustacean aquaculture species. The vitellogenin (Vg) gene and the eyeless (Ey) gene were selected as the targeted genes for mutation. Two sgRNAs were

SUBMITTER: Qiao H 

PROVIDER: S-EPMC10079998 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature