Targeting dormant phenotype acquired mycobacteria using natural products by exploring its important targets: In vitro and in silico studies.

ABSTRACT: The dormant phenotype of Mycobacterium tuberculosis that develops during infection poses a major challenge in disease treatment, since these bacilli show tolerance to front-line drugs. An in vitro hypoxia dormancy model was established, which produced phenotypically dormant Mycobacterium smegmatis after prolonged incubation under conditions of low oxygen, low pH, and nutrient limitation. Bacilli in this model displayed the classical dormancy characters, including loss of acid fastness, altered morphology, and, most importantly, tolerance to front-line drugs. The dormant form of M. smegmatis was treated with drugs and phytomolecules. Three phytomolecules exhibited activity against dormant bacilli, as shown by lack of regrowth in solid and liquid media. Further in

SUBMITTER: Sharma S 

PROVIDER: S-EPMC10080046 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature