Systemic inflammation exacerbates developmental neurotoxicity induced by sevoflurane in neonatal rats.

ABSTRACT:

Background

General anaesthesia in the neonatal period has detrimental effects on the developing mammalian brain. The impact of underlying inflammation on anaesthesia-induced developmental neurotoxicity remains largely unknown.

Methods

Postnatal day 7 (PND7) rats were randomly assigned to receive sevoflurane (3 vol% for 3 h) or carrier gas 12 h after bacterial lipopolysaccharide (LPS; 1 μg g^-1) or vehicle injection. Pharmacological inhibition of caspase-1 by Vx-765 (two doses of 50 μg g^-1 body weight) was used to investigate mechanistic pathways of neuronal injury. Histomorphological injury and molecular changes were quantified 2 h after the end of anaesthesia. Long-term functional deficits were tested at 5-8 weeks of age using a battery of behavioural t

SUBMITTER: Useinovic N 

PROVIDER: S-EPMC10080473 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature