MicroED Structure of a Protoglobin Reactive Carbene Intermediate.

ABSTRACT: Microcrystal electron diffraction (MicroED) is an emerging technique that has shown great potential for describing new chemical and biological molecular structures. Several important structures of small molecules, natural products, and peptides have been determined using ab initio methods. However, only a couple of novel protein structures have thus far been derived by MicroED. Taking advantage of recent technological advances, including higher acceleration voltage and using a low-noise detector in counting mode, we have determined the first structure of an Aeropyrum pernix protoglobin (ApePgb) variant by MicroED using an AlphaFold2 model for phasing. The structure revealed that mutations introduced during directed evolution enhance carbene transfer activity by reorien

SUBMITTER: Danelius E 

PROVIDER: S-EPMC10080679 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature