Multistage Ion Mobility Spectrometry Combined with Infrared Spectroscopy for Glycan Analysis.

ABSTRACT: The structural complexity of glycans makes their characterization challenging, not only because of the presence of various isomeric forms of the precursor molecule but also because the fragments can themselves be isomeric. We have recently developed an IMS-CID-IMS approach using structures for lossless ion manipulations (SLIM) combined with cryogenic infrared (IR) spectroscopy for glycan analysis. It allows mobility separation and collision-induced dissociation of a precursor glycan followed by mobility separation and IR spectroscopy of the fragments. While this approach holds great promise for glycan analysis, we often encounter fragments for which we have no standards to identify their spectroscopic fingerprint. In this work, we perform proof-of-principle experiments employing a multista

SUBMITTER: Bansal P 

PROVIDER: S-EPMC10080682 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature