Effects of Long-Term Storage on the Biobanked Neonatal Dried Blood Spot Metabolome.

ABSTRACT: Over 2.5 million neonatal dried blood spots (DBS) are stored at the Danish National Biobank. These samples offer extraordinary possibilities for metabolomics research, including prediction of disease and understanding of underlying molecular mechanisms of disease development. Nevertheless, Danish neonatal DBS have been little explored in metabolomics studies. One question that remains underinvestigated is the long-term stability of the large number of metabolites typically assessed in untargeted metabolomics over long time periods of storage. Here, we investigate temporal trends of metabolites measured in 200 neonatal DBS collected over a time course of 10 years, using an untargeted liquid chromatography tandem mass spectrometry (LC-MS/MS) based metabolomics protocol. We found that a major

SUBMITTER: Ottosson F 

PROVIDER: S-EPMC10080689 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature