Particle bombardment-assisted peptide-mediated gene transfer for highly efficient transient assay.

ABSTRACT:

Objective

A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells. However, the gene transfer efficiency of CAPT into plant cells was not entirely satisfactory for detecting transient expression of a transgene driven into mitochondria. Here, we report a new gene delivery system using a method called particle bombardment-assisted peptide-mediated gene transfer (PBPT).

Results

We investigated various parameters of the PBPT method to increase transient gene expression efficiency in Brassica campestris. The optimal conditions for PBPT were a single bombardment with gold particles coated with a DNA‒peptide complex (6 µg of DNA and 2 µg of peptide) at an acceleration pressure of 5 kg/cm

SUBMITTER: Kimura M 

PROVIDER: S-EPMC10080836 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature