Multilevel interrogation of H3.3 reveals a primordial role in transcription regulation.

ABSTRACT:

Background

Eukaryotic cells can rapidly adjust their transcriptional profile in response to molecular needs. Such dynamic regulation is, in part, achieved through epigenetic modifications and selective incorporation of histone variants into chromatin. H3.3 is the ancestral H3 variant with key roles in regulating chromatin states and transcription. Although H3.3 has been well studied in metazoans, information regarding the assembly of H3.3 onto chromatin and its possible role in transcription regulation remain poorly documented outside of Opisthokonts.

Results

We used the nuclear dimorphic ciliate protozoan, Tetrahymena thermophila, to investigate the dynamics of H3 variant function in evolutionarily divergent eukaryotes. Functional proteomics and immunofluorescence analyses

SUBMITTER: Nabeel-Shah S 

PROVIDER: S-EPMC10080907 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature