Noninvasive Antemortem Detection of Retinal Prions by a Fluorescent Tracer.

ABSTRACT:

Background

Neurodegenerative diseases are widespread yet challenging to diagnose and stage antemortem. As an extension of the central nervous system, the eye harbors retina ganglion cells vulnerable to degeneration, and visual symptoms are often an early manifestation of neurodegenerative disease.

Objective

Here we test whether prion protein aggregates could be detected in the eyes of live mice using an amyloid-binding fluorescent probe and high-resolution retinal microscopy.

Methods

We performed retinal imaging on an experimental mouse model of prion-associated cerebral amyloid angiopathy in a longitudinal study. An amyloid-binding fluorophore was intravenously administered, and retinal imaging was performed at timepoints corresponding to early, mid-, and terminal pr

SUBMITTER: Aguilar-Calvo P 

PROVIDER: S-EPMC10080909 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature