The miRNA Landscape of Lacrimal Glands in a Murine Model of Autoimmune Dacryoadenitis.

ABSTRACT:

Purpose

To analyze the changes in the lacrimal gland (LG) miRNAome from male nonobese diabetic (NOD) mice with autoimmune dacryoadenitis compared with LG from healthy male BALB/c and dacryoadenitis-free female NOD mice.

Methods

LG from these mice were collected for small RNA sequencing to identify dysregulated miRNAs; hits were validated by RT-qPCR in male NOD and BALB/c LG. Dysregulation of validated species within immune cell-enriched cell fractions and epithelial-enriched cell fractions from LG was probed by RT-qPCR. Ingenuity pathway analysis identified putative miRNA targets, which were examined in publicly available mRNA-seq datasets. Western blotting and confocal imaging of immunofluorescence enabled validation of some molecular changes at the protein level.

Result

SUBMITTER: Singh Kakan S 

PROVIDER: S-EPMC10080918 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature