Comprehensive characterization of a transgene insertion in a highly repetitive, centromeric region of Anopheles mosquitoes.

ABSTRACT: The availability of the genomic sequence of the malaria mosquito Anopheles gambiae has in recent years sparked the development of transgenic technologies with the potential to be used as novel vector control tools. These technologies rely on genome editing that confer traits able to affect vectorial capacity. This can be achieved by either reducing the mosquito population or by making mosquitoes refractory to the parasite infection. For any genetically modified organism that is regarded for release, molecular characterization of the transgene and flanking sites are essential for their safety assessment and post-release monitoring. Despite great advancements, Whole-Genome Sequencing data are still subject to limitations due to the presence of repetitive and unannotated DNA sequences.

SUBMITTER: Vitale M 

PROVIDER: S-EPMC10081084 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature