Long-term evolution of proliferating cells using the eVOLVER platform.

ABSTRACT: Experimental evolution using fast-growing unicellular organisms is a unique strategy for deciphering the principles and mechanisms underlying evolutionary processes as well as the architecture and wiring of basic biological functions. Over the past decade, this approach has benefited from the development of powerful systems for the continuous control of the growth of independently evolving cultures. While the first devices compatible with multiplexed experimental evolution remained challenging to implement and required constant user intervention, the recently-developed eVOLVER framework represents a fully automated closed-loop system for laboratory evolution assays. However, it remained difficult to maintain and compare parallel evolving cultures in tightly controlled environments over lon

SUBMITTER: Garcia-Ruano D 

PROVIDER: S-EPMC10081182 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature