Placental Cell Conditioned Media Modifies Hematopoietic Stem Cell Transcriptome In Vitro.

ABSTRACT:

Background

Hematopoietic stem cells are cells that differentiate into all blood cell types. Although the placenta secretes hormones, proteins and other factors important for maternal and fetal health, cross-talk between placental cells and hematopoietic stem cells is poorly understood. Moreover, toxicant impacts on placental-hematopoietic stem cell communication is understudied. The goals of this study were to determine if factors secreted from placental cells alter transcriptomic responses in hematopoietic stem cells and if monoethylhexyl phthalate (MEHP), the bioactive metabolite of the pollutant diethylhexyl phthalate, modifies these effects.

Methods

We used K-562 and BeWo cells as in vitro models of hematopoietic stem cells and placental syncytiotrophoblasts, resp

SUBMITTER: Harris SM 

PROVIDER: S-EPMC10081206 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature