Confocal interferometric scattering microscopy reveals 3D nanoscopic structure and dynamics in live cells.

ABSTRACT: Bright-field light microscopy and related phase-sensitive techniques play an important role in life sciences because they provide facile and label-free insights into biological specimens. However, lack of three-dimensional imaging and low sensitivity to nanoscopic features hamper their application in many high-end quantitative studies. Here, we demonstrate that interferometric scattering (iSCAT) microscopy operated in the confocal mode provides unique label-free solutions for live-cell studies. We reveal the nanometric topography of the nuclear envelope, quantify the dynamics of the endoplasmic reticulum, detect single microtubules, and map nanoscopic diffusion of clathrin-coated pits undergoing endocytosis. Furthermore, we introduce the combination of confocal and wide-field iSCAT modalit

SUBMITTER: Kuppers M 

PROVIDER: S-EPMC10081331 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature