Nanopore-based disease diagnosis using pathogen-derived tryptic peptides from serum.

ABSTRACT: Nanopore sensors have shown great utility in nucleic acid detection and sequencing approaches. Recent studies also indicate that current signatures produced by peptide-nanopore interactions can distinguish high purity peptide mixtures, but the utility of nanopore sensors in clinical applications still needs to be explored due to the inherent complexity of clinical specimens. To fill this gap between research and clinical nanopore applications, we describe a methodology to select peptide biomarkers suitable for use in an immunoprecipitation-coupled nanopore (IP-NP) assay, based on their pathogen specificity, antigenicity, charge, water solubility and ability to produce a characteristic nanopore interaction signature. Using tuberculosis as a proof-of-principle example in a disease that can b

SUBMITTER: Zheng W 

PROVIDER: S-EPMC10081497 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature