Identification of Human Host Substrates of the SARS-CoV-2 M^pro and PL^pro Using Subtiligase N-Terminomics.

ABSTRACT: The recent emergence of SARS-CoV-2 in the human population has caused a global pandemic. The virus encodes two proteases, M^pro and PL^pro, that are thought to play key roles in the suppression of host protein synthesis and immune response evasion during infection. To identify the specific host cell substrates of these proteases, active recombinant SARS-CoV-2 M^pro and PL^pro were added to A549 and Jurkat human cell lysates, and subtiligase-mediated N-terminomics was used to capture and enrich protease substrate fragments. The precise location of each cleavage site was identified using mass spectrometry. Here, we report the identification of over 200 human host proteins that are potential substrates for SARS-CoV-2 M^pro and PL^pro

SUBMITTER: Luo SY 

PROVIDER: S-EPMC10081575 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature