Microfluidic Systems with Embedded Cell Culture Chambers for High-Throughput Biological Assays.

ABSTRACT: The ability to generate chemical and mechanical gradients on chips is important for either creating biomimetic designs or enabling high-throughput assays. However, there is still a significant knowledge gap in the generation of mechanical and chemical gradients in a single device. In this study, we developed gradient-generating microfluidic circuits with integrated microchambers to allow cell culture and to introduce chemical and mechanical gradients to cultured cells. A chemical gradient is generated across the microchambers, exposing cells to a uniform concentration of drugs. The embedded microchamber also produces a mechanical gradient in the form of varied shear stresses induced upon cells among different chambers as well as within the same chamber. Cells seeded within the chambers rem

SUBMITTER: Jaberi A 

PROVIDER: S-EPMC10081828 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature