Ablation of TRPC3 disrupts Ca²⁺ signaling in salivary ductal cells and promotes sialolithiasis.

ABSTRACT: Clinical studies and structural analyses of salivary stones strongly suggest a linkage between higher saliva calcium (Ca²⁺) and salivary stone formation, sialolithiasis; however, the process and the mechanism leading to Ca²⁺ overload during sialolithiasis is not well understood. Here, we show that TRPC3 null (-/-) mice presented with a reduction in Ca²⁺ entry and current in ductal cells with higher saliva [Ca²⁺] suggesting diminished transepithelial Ca²⁺ flux across the salivary ductal cells, leaving more Ca²⁺ in ductal fluid. Significantly, we found that TRPC3 was expressed in mice and human salivary ductal cells, while intraductal stones were detected in both mice (TRPC3^-/-) and patient (sialolithiasis) salivary

SUBMITTER: Choi BE 

PROVIDER: S-EPMC10082769 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature