Engineering yeast mitochondrial metabolism for 3-hydroxypropionate production.

ABSTRACT:

Background

With unique physiochemical environments in subcellular organelles, there has been growing interest in harnessing yeast organelles for bioproduct synthesis. Among these organelles, the yeast mitochondrion has been found to be an attractive compartment for production of terpenoids and branched-chain alcohols, which could be credited to the abundant supply of acetyl-CoA, ATP and cofactors. In this study we explored the mitochondrial potential for production of 3-hydroxypropionate (3-HP) and performed the cofactor engineering and flux control at the acetyl-CoA node to maximize 3-HP synthesis.

Results

Metabolic modeling suggested that the mitochondrion serves as a more suitable compartment for 3-HP synthesis via the malonyl-CoA pathway than the cytosol, due to the oppo

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC10082987 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature