A cationic motif upstream Engrailed2 homeodomain controls cell internalization through selective interaction with heparan sulfates.

ABSTRACT: Engrailed2 (En2) is a transcription factor that transfers from cell to cell through unconventional pathways. The poorly understood internalization mechanism of this cationic protein is proposed to require an initial interaction with cell-surface glycosaminoglycans (GAGs). To decipher the role of GAGs in En2 internalization, we have quantified the entry of its homeodomain region in model cells that differ in their content in cell-surface GAGs. The binding specificity to GAGs and the influence of this interaction on the structure and dynamics of En2 was also investigated at the amino acid level. Our results show that a high-affinity GAG-binding sequence (RKPKKKNPNKEDKRPR), upstream of the homeodomain, controls En2 internalization through selective interactions with highly-sulfated heparan su

SUBMITTER: Cardon S 

PROVIDER: S-EPMC10083169 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature