Single-cell RNA sequencing reveals in vivo osteoimmunology interactions between the immune and skeletal systems.

ABSTRACT:

Background

While osteoimmunology interactions between the immune and skeletal systems are known to play an important role in osteoblast development, differentiation and bone metabolism related disease like osteoporosis, such interactions in either bone microenvironment or peripheral circulation in vivo at the single-cell resolution have not yet been characterized.

Methods

We explored the osteoimmunology communications between immune cells and osteoblastic lineage cells (OBCs) by performing CellphoneDB and CellChat analyses with single-cell RNA sequencing (scRNA-seq) data from human femoral head. We also explored the osteoimmunology effects of immune cells in peripheral circulation on skeletal phenotypes. We used a scRNA-seq dataset of peripheral blood monocytes (PBMs)

SUBMITTER: Wang S 

PROVIDER: S-EPMC10083244 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature