Effects of aging and macrophages on mice stem Leydig cell proliferation and differentiation in vitro.

ABSTRACT:

Background

Testosterone plays a critical role in maintaining reproductive functions and well-beings of the males. Adult testicular Leydig cells (LCs) produce testosterone and are generated from stem Leydig cells (SLCs) during puberty through adulthood. In addition, macrophages are critical in the SLC regulatory niche for normal testicular function. Age-related reduction in serum testosterone contributes to a number of metabolic and quality-of-life changes in males, as well as age-related changes in immunological functions. How aging and testicular macrophages may affect SLC function is still unclear.

Methods

SLCs and macrophages were purified from adult and aged mice via FACS using CD51 as a marker protein. The sorted cells were first characterized and then co-culture

SUBMITTER: Shao J 

PROVIDER: S-EPMC10083278 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature