Toward high-throughput engineering techniques for improving CAR intracellular signaling domains.

ABSTRACT: Chimeric antigen receptors (CAR) are generated by linking extracellular antigen recognition domains with one or more intracellular signaling domains derived from the T-cell receptor complex or various co-stimulatory receptors. The choice and relative positioning of signaling domains help to determine chimeric antigen receptors T-cell activity and fate in vivo. While prior studies have focused on optimizing signaling power through combinatorial investigation of native intracellular signaling domains in modular fashion, few have investigated the prospect of sequence engineering within domains. Here, we sought to develop a novel in situ screening method that could permit deployment of directed evolution approaches to identify intracellular domain variants that drive selective in

SUBMITTER: Butler SE 

PROVIDER: S-EPMC10083361 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature