Accurate isolation and detection of circulating tumor cells using enrichment-free multiparametric high resolution imaging.

ABSTRACT:

Introduction

The reliable and accurate detection of rare circulating tumor cells (CTCs) from cancer patient blood samples promises advantages in both research and clinical applications. Numerous CTC detection methods have been explored that rely on either the physical properties of CTCs such as density, size, charge, and/or their antigen expression profiles. Multiple factors can influence CTC recovery including blood processing method and time to processing. This study aimed to examine the accuracy and sensitivity of an enrichment-free method of isolating leukocytes (AccuCyte^® system) followed by immunofluorescence staining and high-resolution imaging (CyteFinder^® instrument) to detect CTCs.

Method

Healthy human blood samples, spiked with cancer cells f

SUBMITTER: Yeo D 

PROVIDER: S-EPMC10083432 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature