Multiple Leaf Sample Extraction System (MuLES): A tool to improve automated morphometric leaf studies.

ABSTRACT:

Premise

The measurement of leaf morphometric parameters from digital images can be time-consuming or restrictive when using digital image analysis softwares. The Multiple Leaf Sample Extraction System (MuLES) is a new tool that enables high-throughput leaf shape analysis with minimal user input or prerequisites, such as coding knowledge or image modification.

Methods and results

MuLES uses contrasting pixel color values to distinguish between leaf objects and their background area, eliminating the need for color threshold-based methods or color correction cards typically required in other software methods. The leaf morphometric parameters measured by this software, especially leaf aspect ratio, were able to distinguish between large populations of different accessions for th

SUBMITTER: Bowman CS 

PROVIDER: S-EPMC10083438 | biostudies-literature | 2023 Mar-Apr

REPOSITORIES: biostudies-literature