Infrared spectroscopy for ploidy estimation: An example in two species of Veronica using fresh and herbarium specimens.

ABSTRACT:

Premise

Polyploidy has become a central factor in plant evolutionary biological research in recent decades. Methods such as flow cytometry have revealed the widespread occurrence of polyploidy; however, its inference relies on expensive lab equipment and is largely restricted to fresh or recently dried material.

Methods

Here, we assess the applicability of infrared spectroscopy to infer ploidy in two related species of Veronica (Plantaginaceae). Infrared spectroscopy relies on differences in the absorbance of tissues, which could be affected by primary and secondary metabolites related to polyploidy. We sampled 33 living plants from the greenhouse and 74 herbarium specimens with ploidy known through flow cytometrical measurements and analyzed the resulting spectra usi

SUBMITTER: Buono D 

PROVIDER: S-EPMC10083463 | biostudies-literature | 2023 Mar-Apr

REPOSITORIES: biostudies-literature