An immunoresponsive three-dimensional urine-tolerant human urothelial model to study urinary tract infection.

ABSTRACT:

Introduction

Murine models of urinary tract infection (UTI) have improved our understanding of host-pathogen interactions. However, given differences between rodent and human bladders which may modulate host and bacterial response, including certain biomarkers, urothelial thickness and the concentration of urine, the development of new human-based models is important to complement mouse studies and to provide a more complete picture of UTI in patients.

Methods

We originally developed a human urothelial three-dimensional (3D) model which was urine tolerant and demonstrated several urothelial biomarkers, but it only achieved human thickness in heterogenous, multi-layered zones and did not demonstrate the comprehensive differentiation status needed to achieve barrier function.

SUBMITTER: Jafari NV 

PROVIDER: S-EPMC10083561 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature