Subtle sequence differences between two interacting σ⁵⁴ -dependent regulators lead to different activation mechanisms.

ABSTRACT: In the strictly anaerobic nitrate reducing bacterium Aromatoleum anaerobium, degradation of 1,3-dihydroxybenzene (1,3-DHB, resorcinol) is controlled by two bacterial enhancer-binding proteins (bEBPs), RedR1 and RedR2, which regulate the transcription of three σ⁵⁴ -dependent promoters controlling expression of the pathway. RedR1 and RedR2 are identical over their length except for their N-terminal tail which differ in sequence and length (six and eight residues, respectively), a single change in their N-terminal domain (NTD), and nine non-identical residues in their C-terminal domain (CTD). Their NTD is composed of a GAF and a PAS domain connected by a linker helix. We show that each regulator is controlled by a different mechanism: whilst RedR1 responds to the classical NTD-medi

SUBMITTER: Pacheco-Sanchez D 

PROVIDER: S-EPMC10084136 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature