Urinary extracellular vesicles as a source of protein-based biomarkers in feline chronic kidney disease and hypertension.

ABSTRACT:

Objectives

To validate a methodology for isolating feline urinary extracellular vesicles and characterise the urinary extracellular vesicle population and proteome in cats with normal renal function and cats with normotensive or hypertensive chronic kidney disease.

Methods

Feline urinary extracellular vesicles were isolated using three different methods (precipitation alone, precipitation followed by size exclusion chromatography and ultrafiltration followed by size exclusion chromatography, which were compared via transmission electron microscopy and nanoparticle tracking analysis. Cats with normal renal function (n=9), normotensive chronic kidney disease (n=10) and hypertensive chronic kidney disease (n=9) were identified and urinary extracellular vesicles isolated from pa

SUBMITTER: Lawson JS 

PROVIDER: S-EPMC10084206 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature