Conditioned serum in vitro treatment of chondrocyte pellets and osteoarthritic explants.

ABSTRACT:

Background

Autologous conditioned serum (ACS) is used to treat osteoarthritis in horses, although its effects are not fully investigated.

Objectives

To investigate the effects of equine serum and conditioned serum on chondrocytes stimulated with interleukin (IL)-1β and cartilage explants with mild osteoarthritis.

Study design

In vitro experimental study.

Methods

The effect of three different serum preparations (unincubated control [PS], serum incubated 24 h [PS24h] and serum incubated 24 h in ACS containers [PCS]) pooled from lame horses were tested in two in vitro models. IL-1β and IL-1 receptor antagonist (IL-1Ra) concentrations were measured in all sera. In model 1, chondrocyte pellet cultures were stimulated with IL-1β prior to treatment with the serum prep

SUBMITTER: Lofgren M 

PROVIDER: S-EPMC10084271 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature