Automated fluorescence gating and size determination reduce variation in measured concentration of extracellular vesicles by flow cytometry.

ABSTRACT: Extracellular vesicles (EVs) are an upcoming biomarker for disease. However, the measured concentrations of EVs by flow cytometry are incomparable due to analytical variables. This study aimed to investigate how the choice of fluorophore, and thereby brightness, affects the measured concentration of EVs. Four commonly used fluorophores allophycocyanin, Brilliant Violet-421, fluorescein isothiocyanate, and phycoerythrin, all conjugated to CD61 antibodies, were used to label platelet-derived extracellular vesicles (PEVs) in human plasma. PEVs were measured by flow cytometry. The concentration of EVs was obtained by manually set fluorescence gates, automatically determined fluorescence gates, and automatically determined fluorescence gates combined with specific size gates. Manually set fluor

SUBMITTER: Gankema AAF 

PROVIDER: S-EPMC10084316 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature