Multiplex Immunofluorescence Image Quality Checking Using DAPI Channel-referenced Evaluation.

ABSTRACT: Multiplex immunofluorescence (MxIF) images provide detailed information of cell composition and spatial context for biomedical research. However, compromised data quality could lead to research biases. Comprehensive image quality checking (QC) is essential for reliable downstream analysis. As a reliable and specific staining of cell nuclei, 4',6-diamidino-2-phenylindole (DAPI) signals were used as references for tissue localization and auto-focusing across MxIF staining-scanning-bleaching iterations and could potentially be reused for QC. To confirm the feasibility of using DAPI as QC reference, pixel-level DAPI values were extracted to calculate signal fluctuations and tissue content similarities in staining-scanning-bleaching iterations for identifying quality issues. Concordance between

SUBMITTER: Jiang J 

PROVIDER: S-EPMC10084566 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature