Improvement of Bacilysin Production in <i>Bacillus subtilis</i> by CRISPR/Cas9-Mediated Editing of the 5'-Untranslated Region of the <i>bac</i> Operon.

ABSTRACT: Bacilysin is a dipeptide antibiotic composed of L-alanine and L-anticapsin produced by certain strains of Bacillus subtilis. Bacilysin is gaining increasing attention in industrial agriculture and pharmaceutical industries due to its potent antagonistic effects on various bacterial, fungal, and algal pathogens. However, its use in industrial applications is hindered by its low production in the native producer. The biosynthesis of bacilysin is mainly based on the bacABCDEF operon. Examination of the sequence surrounding the upstream of the bac operon did not reveal a clear, strong ribosome binding site (RBS). Therefore, in this study, we aimed to investigate the impact of RBS as a potential route to improve bacilysin production. For this, the 5' untranslated region (5'

SUBMITTER: Abdulmalek HW 

PROVIDER: S-EPMC10084748 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature