Epifluorescence Microscopy with Image Analysis as a Promising Method for Multispecies Biofilm Quantification.

ABSTRACT: Epifluorescence microscopy with image analysis was evaluated as a biofilm quantification method (i.e., quantification of surface area colonized by biofilms), in comparison with crystal violet (CV) staining. We performed different experiments to generate multispecies biofilms with natural and artificial bacterial assemblages. First, four species were inoculated daily in 16 different sequences to form biofilms (surface colonization, 0.1%-56.6%). Second, a 9-species assemblage was allowed to form biofilms under 10 acylase treatment episodes (33.8%-55.6%). The two methods comparably measured the quantitative variation in biofilms, exhibiting a strong positive relationship (R² ≥ 0.7). Moreover, the two methods exhibited similar levels of variation coefficients. Finally,

SUBMITTER: Lee JW 

PROVIDER: S-EPMC10084751 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature