Transcriptional Interplay between Malassezia restricta and Staphylococcus Species Co-Existing in the Skin Environment.

ABSTRACT: Malassezia and Staphylococcus are the most dominant genera in human skin microbiome. To explore the inter-kingdom interactions between the two genera, we examined the transcriptional changes in Malassezia and Staphylococcus species induced upon co-culturing. RNA-seq analyses revealed that genes encoding ribosomal proteins were upregulated, while those encoding aspartyl proteases were downregulated in M. restricta after co-culturing with Staphylococcus species. We identified MRET_3770 as a major secretory aspartyl protease coding gene in M. restricta through pepstatin-A affinity chromatography followed by mass spectrometry and found that the expression of MRET_3770 was significantly repressed upon co-culturing with Staphylococcus speci

SUBMITTER: Yang HO 

PROVIDER: S-EPMC10084760 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature