Recipient UvrD helicase is involved in single- to double-stranded DNA conversion during conjugative plasmid transfer.

ABSTRACT: Dissemination of antibiotic resistance, a current societal challenge, is often driven by horizontal gene transfer through bacterial conjugation. During conjugative plasmid transfer, single-stranded (ss) DNA is transferred from the donor to the recipient cell. Subsequently, a complete double-stranded (ds) plasmid molecule is generated and plasmid-encoded genes are expressed, allowing successful establishment of the transconjugant cell. Such dynamics of transmission can be modulated by host- or plasmid-encoded factors, either in the donor or in the recipient cell. We applied transposon insertion sequencing to identify host-encoded factors that affect conjugative transfer frequency in Escherichia coli. Disruption of the recipient uvrD gene decreased the acquisition frequency of conjugative pl

SUBMITTER: Shen M 

PROVIDER: S-EPMC10085688 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature