A genome-wide map of DNA replication at single-molecule resolution in the malaria parasite Plasmodium falciparum.

ABSTRACT: The malaria parasite Plasmodium falciparum replicates via schizogony: an unusual type of cell cycle involving asynchronous replication of multiple nuclei within the same cytoplasm. Here, we present the first comprehensive study of DNA replication origin specification and activation during Plasmodium schizogony. Potential replication origins were abundant, with ORC1-binding sites detected every ∼800 bp. In this extremely A/T-biased genome, the sites were biased towards areas of higher G/C content, and contained no specific sequence motif. Origin activation was then measured at single-molecule resolution using newly developed DNAscent technology: a powerful method of detecting replication fork movement via base analogues in DNA sequenced on the Oxford Nanopore platform. Unusually, origins we

SUBMITTER: Totanes FIG 

PROVIDER: S-EPMC10085703 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature